MANAGING INTERNATIONAL OPERATIONS Essay Example | Topics and Well Written Essays - 1000 words

MANAGING INTERNATIONAL OPERATIONS - Essay Example Most of the EU trading occurs in between the European countries itself, owing to its flourishing nature it stands first among exporting and second among importing countries. The key concepts of European Union are, Integration- combining a range of different pieces into a single body, or the removal of barriers to enable the pieces to move closer together (Jones 2008,pp.88-87), Supranationalism – which express the power of EU decisions that override those made by national governments whenever the two conflict (Ibid ,pp.137-138) and the Intergovernmentalism – the idea of different governments of EU working together to protect its national interests (Ibid 2008,p.87). The most important key institutions of European Union are The council of the European Union representing the governments of the member states; European commission which consists of the commissioners appointed by the member states ,the executive body and driving force ; The European Council that comprises the h eads of state/government of the member states which gives political direction to the EU and sets policy agenda and strategies for the EU and European Parliament elected by the people of the member state, law-making body. ... n spreads some precious lessons to the whole world through their integrated efforts and activities which if put into effect may enable all human beings to live in peace and harmony around the Globe. References Jones, A. (2008) A Glossary of the European Union. Edinburgh University Press. The EU & Supply Chain Management The Supply Chain Management (SCM) can be defined as the† management of upstream and downstream relationship with suppliers and customers in order to deliver superior customer value at less cost to the supply chain† which refers to a system of organizations in moving a product from supplier to the customer (Christopher, 2011, p. 3). SCM heavily depends on the areas of operation management, logistics, procurement, and information technology (Ibid). Some suggest it is more accurate to use the term â€Å"Supply network†, viewing the supply chain as â€Å"a network of connected and interdependent organizations mutually and cooperatively working together to control, manage and improve the flow of materials and information from suppliers to end users† (Chopra & Meindl 2010). Different SCM models were proposed for the better understanding and systematic coordination of the business functions. For instance, Supply Chain Council promotes Supply Chain Operations Reference (SCOR) whereas the Global Supply Chain Forum (GSCF) promotes SCM Model. By going truly global with the SCM, business firms can understand the competitiveness and can identify significant links in the network. It also helps to focus on long term issues. According to Chopra and Meindl (2010), the decision phases of a supply chain includes 3 steps the Supply Chain Strategy or design, Supply Chain Planning and the Supply Chain Operations. Essentially the fundamental role of SCM is to facilitate the

European Business Environment Essay Example for Free

European Business Environment Essay Introduction This paper focuses on the enlargement of the European Union (EU) and its effects on external and internal relations. Taking into account the analysis and forecast presented before enlargement, it shows the real consequences of the development. The most essential consequences, as well as problems in part of decision-making and administration in the EU, social consequences of the enlargement, as well as the result of the possible economic enlargement of the on both existing member countries and new entrant countries. In taking into account the future of the European Union and the perception of regional European, the study specify that for the first time in many decades the EU has the opportunity to strengthen the global role of the society and reunify the continent supported by a common democratic standards and rules, a collapse in the process of European integration would mainly perhaps mean the gradual marginalization of Europe as a foremost actor of international associations. The collapse of the Soviet Union and the fall of the bipolar order greatly affected the development of European integration. One of the main basic challenges was the disappearance of the Eastern Bloc, as the threat of the Soviet hostility served for many years as a amalgamate aspect for the Western community (Baun, 2004). The next challenge was the unification of Germany, as it was obvious for most of European countries that the prospect role and position of the united German state would be stronger and further important than that of Cold War-era Western Germany. At the same illustration, the European Union, formally established by the Treaty of Maastricht on 1 November 1993, had to respond to broader problems about its international target and the future shape. It was apparent that the European Union possibly could not have opened its door to nation that was unwary or unable to prove the good organization of their democratic governance. Additionally, the setback for the EU was that planed to outlook for the relationship served as encouragement for the new democratic organization in East-Central Europe to carry on their complex and socially troublesome reforms, the flourishing results of which became significant for the strength of the whole continent. The solution was the Copenhagen condition, explained at the summit of the European Council in Copenhagen in 1993. The rules placed general necessities for starting effective democratic organizations, respect for individual and minority human rights, and suitable instruments for promised market economy (Lindner, 2003).Upon meeting the requirement, the first candidates was capable of open the accession talks in 1998. Ten new members that united the European Union on 1 May 2004 completed these consultations in 2002. Jointly with the growth in 2004, the EU-15 developed into EU-25; after Bulgarian and Romanian accession on 1 January 2007, the coming together became the EU-27. The growth from 15 to 27 member states was the biggest in the history of European incorporation process growing the number of the EU population from about 380 to 485 million. The new states members were medium-sized and small countries, though; each of the new associate has achieved the same rights as existing members of the EU. The enlargement of Eastern has been the major difficulties in the history of European integration, not simply because of the number of new states member joining the European Union at the same time, but mainly because of differences in the level of the gross national product (GNP) involving the old and the new members states of the EU. An evaluation of GNP per capita confirms that the richest new members state have not go beyond 40% of the standard EU-15 level and much bigger asymmetry is opened by the evaluation with the wealthiest states members of the old union (Miles, 2004). In reality, earlier enlargement rounds, like the one in 1973, to comprise the Ireland, Denmark and United Kingdom the one in 1995, to include Austria, Finland, and Sweden, was accessions of states similar in economic improvement and wealth. Merely the membership of Spain and Greece in 1981 and Portugal in 1986 caught up the enlargements of countries, which were much not as good as at the moment of their accession than the standard member of the society. This led to a question of harmony between the poorer members and the rich, and needed additional financial contribution of the wealthiest member states to sustain political and economic transformation in the new member countries. Although the old member countries reacted with hesitation, they finally agreed to such assistance, being aware that it would support democratic transformation on the Continent and support to eliminate intimidation of instability for the whole society, while contributing to formation of the common European marketplace. Nevertheless the Eastern enlargement twenty years later on was incomparable in its unevenness of economic potentials and the interns of GNP per capita involving the old and the new members still with the Southern growth of the European Community (EC) in the mid-1980s (Nugent, 2004). This irregularity of enlargement collectively with fears in Western Europe about social cost, problems of intra-union administration as well as the continuing crises of EU characteristics have compounded the face of the Eastern enlargement years after the enlargement, these concern continue to distress the integration development, consequently it is worth focusing on a few of them. Even ahead of agreement, it was obvious that the Eastern enlargement from 15 to 28 members would force management and decision-making processes in the European Union, as well as the possible to paralyze or at least make difficult the mechanisms formed in the EU-15. Predicting the enlargement, the old members attempted to organize the internal decision-making systems to incorporate the new members. The Amsterdam and Nice Treaties broaden the scope of verdict to be taken supported on Qualified Majority Voting (QMV), as an alternative of harmony, and the Nice Treaty formed a specific majority system (Schmitter, 2004). It established each of the 27 members an exact number of votes gleaming its demographical potential. The major members of the EU France, Germany, Italy and the UK, were awarded 29 votes each, and the smallest states: Luxembourg 3 and Malta 4 votes, correspondingly. Poland, as the largest new member, was given 27 votes, the equivalent to the number as Spain. However, the rule of the accord has remained at as the foundation of all decision-making in the EU, particularly when it comes to critical political decisions. Even so, the caution of Taylor remained applicable as the effectiveness of decision-making in the distended European Union needed further internal amendment. The response was Treaty making a Constitution for Europe signed in Rome on 29 October 2004. The new agreement went comparatively far in its application, yet Netherlands and the France rejected the European Constitution in referendums and the last effort to improve the internal utility of the EU was the Lisbon Treaty signed on 13 December 2007. The Lisbon Treaty is in actual fact a series of provisions originally presented in the European Constitution. It has make things easier for the   EU’s legal procedures and has established the European Union a legal personality, as well as helping harmonization of the EU’s policies establishing the posts of the EU President and Foreign Minister. It reflects the objections of some members who strained the principal independence of the member states, resultant in the cut of most references that could advocate for the character of the European Union as a (super-) state, counting the initial names of the new EU council. As an effect, the new EU Foreign Minister has lastly become the High Representative of the Union for Foreign Affairs and Security Policy. However, the prospect of the treaty had been vague for almost two years, as its implementation required confirmation in all member states. After the optimistic result of the second Irish referendum on 2 October 2009, the president of the Czech Republic, the Polish president, Vaclav Klaus and Lech Kaczynski, finally decided to sign the Treaty. This permitted the Lisbon Treaty to come in into force on 1 December 2009 (Nugent, 2004a). The approach of the citizens in the new member states towards European incorporation has generally been optimistic. For instance, the effects of the agreement referendum in Poland demonstrated support for the association at 77%. After enlargement, assistance in Poland is still at a high level of about 70% (Preston, 1997). The Office of the Committee for European Integration, Warsaw 2009 all together, still, enlargement provoked serious worries among Western EU member countries. The forecast of the enlargement were a basis of anxiety among the populace in the old, wealthier counties members of the EU, as well as the fears of joblessness and huge immigration of employees from the much poorer states of East-Central Europe. Piotr M. Kaczynski specified that the new states were better organized for enlargement as they projected changes and were required to plan for membership. The older members and their societies only experienced the test of the Eastern enlargement on the day of new members’ agreement (Nugent, 2004b). This resulted in the distribution of fears and slogans about the â€Å"Polish nurse† or â€Å"Polish plumber† frightening for employment chances for local Western Europeans. Few in Western Europe left devoid of the benefits of the Eastern enlargement for stabilization, democratization, and union of the continent. Yet the Westerners worried that Eastern enlargement would establish   the questions of decision-making, management, asymmetry in economic potentials and as well as raise the requirement for a broader process of structuring mutual consideration between the two share equally of Europe divided for almost 50 years by the â€Å"Iron Curtain.† (Hagemann, Sara/De, Julia 2007) Still, there were numerous forecasts concerning the migration prospective. Some of them projected that the immigration from the new states would differ from 6% to even 30% of their total populace (105 million), but those more practical showed that the migration would be approximately 3-5% or yet only 2% in the longer time, taking return migration into consideration (Nugent, 2004b). In actuality, the Eastern enlargement has only in part established the fears of Western European civilization and its social effects have been less serious than projected. At the same time, it has established most of the scholars’ forecasts. In agreement with the data obtainable by Euro stat for the end of 2008, the total digit of the immigrants from the new states members has been approximately 1.7 million (Guà ©rot, 2005).This does not comprise session seasonal personnel, the number of which could be expected as an additional few hundred thousand with a propensity to sluggish down for the reason that of the current world economic disaster. The most frequent immigrants were Poles (Poland being the most crowded new member state) and Romanians operating mostly in the Ireland, Spain, and United Kingdom. Polish specialist have projected that the actual number of Poles operating in the old member countries of the EU has been at the level of 1–1.12 million (out of entirety 38 million of populace) with a possible of additional 0.6 million of seasonal recruits (Stacey, and Berthold 2003). These numbers may seem considerable, but it would be difficult to treat them as a massive influx of workers from the East. All in all, the trouble of migration from the new state member cannot be overlooked and will go on to play an important role in the opinion of euro-skeptics, but it emerge to be much less grave than originally estimated. This is accurate mainly in the situation of the current world economic catastrophe and increasing social protests adjacent to the foreign employment force in the UK and other principal states of the European Union. It may perhaps also persuade the old members to formulate informal efforts at protectionism, although the regulations of the free movement of industry and capital within the European particular market. One of the areas of debate before enlargement was the cost of the process. Yet it seems that the Eastern enlargement of the European Union has not been overly expensive, especially in comparison to the benefits. Several authors have concluded that in the longer term the enlargement would have small but positive growth effects on the whole EU, although lower in the case of the old members and higher with regard to new member states, with forecasts of an additional overall EU growth by 0.5 to 0.7%. However, it is clear that the main benefits of the enlargement are political ones, namely the opportunity to reunify the Continent and in this context, the costs of the enlargement were indeed low (estimated for the financial framework 2004-2006 at 40.16 billion euro, or 1.08% of EU GNP) (Faber, 2009). Though, looking at the differences in economic prospective and wealth-level involving the old states and new states members, it seems not possible to expect that their convergence would be attained in the short or even medium time. This implies a tough need for aiding funds from the richest states of the European Union for the improvement of the new state members. Therefore, the dilemma of financial unity between the poorest and the richest in the EU will turn into much more grave for the future of the Union than whichever time before, still after the Southern enlargement in the 1980s (Falkner, 1996). The narrow financial wealth at the Union’s disposal has led to discussion in the EU about its financial point of view. The member states take up two differing positions. France and Germany, as the leading donors, have grown hesitant to increase their assistance to the common EU budget. The financial discussions for the period 2007-2013 incorporated the demand of the major net-contributors to edge the EU resources to 1% of the Community’s GNP. The ultimate resolution accepted a maximum of 1.045%, but the indecision of the richest members to wrap the additional expenditure of the enlarged EU was understandable. The new state members, conversely, joined the EU with the hope of financial support and harmony. Knowing that their active growth depend on the kindness of the richest states of the EU, the new state fear that, the imposing idea of harmony might crash with the exaction economic interests of the main EU members. Such a distribution of the EU members into the center of the most urbanized and wealthiest nations and East-Central Europe as an edge could intimidate European integration. Yet even though the limited economic resources exist in the EU budget, the supporting of the new members states has been reasonable so far. Poland, for instance, as the largest recipient, is getting a net-assistance of â‚ ¬60 billion in the episode of 2007-2013, mostly in the form of structural and solidity funds. However, the most important concern is to keep this level of the help in the next financial agenda (2014-2020). This will be the main issue of the future discussion. The wealthiest and strongest states of the EU have well thought-out reducing some forms of support to new member’s states and support spending that would center more on new technologies, competitiveness, and innovation of the EU in the global economy. Even if it is apparent that the EU requires being more successful on the global prospect, it is evident that the financial capital available in the upcoming EU budget for innovation and new technologies would most possibly help the wealthiest state of the Union. The new states with their much poorer economies and less innovative will not be capable to struggle for this money. The new East-Central European members projected full-fledged contribution in the decision-making method of the EU, as well as value for their opinion, despite their imperfect economic potentials. But the political discussion just after the Eastern extension, which led into the new suggestion of the European Constitution and the Lisbon Treaty, were challenging for the new members states and tackle them with the perception of the new form of the European Union only a few months following their agreement. These circumstances were most difficult for Poland the largest country in the group of new states, however a medium-size state members in the EU with its ambition of playing an active political task in the European Union. In the case of Poland, which was to lower some of its recognized position in the EU exacted in the number of votes in a number of Union institutions, it was hard to clarify to people why the regulations of the membership must be altered so quickly after the agreement. Examining the first epoch after the Eastern extension, Piotr M. Kaczynski sustains that the economic outcome of the growth have been clearly constructive. The new state members improved quickly and much earlier than expected. Though, he finds that the political aspect of the enlargement is harder to review. After a first period of compliance, the new state members, particularly Czech Republic and the Poland, became more self-confident in the EU, which resulted to some quarrel between the administration of these nations and EU system. Equally Czech Republic and the Poland mainly reacted to the thoughts coming from the Western part of the EU. Their political program, if any, were typically poorly set and cast off (Steunenberg, 2002a). Later, after the enlargement, Poland and all new members comprehend more visibly that it is not only the amount of votes that make a decision their place and ability to pressure decision-making procedures in the EU. They have attained convenient experience and become more familiar with the actual political device, including the regulations of effective alliance building and cooperation. Dirk Leuffen has explained it as a progression of â€Å"socialization† in which the new state members learn how to deal with the informal and formal rules and standards in the EU. From his perspective point of view, this socialization should be well thought-out as a medium-term development ( Dirk 2010). The skill gained; during the five years following the enlargement appear to back up that the time of socialization will be shorter relatively than longer. The current Polish-Swedish suggestion of Eastern Partnership (Steunenberg, 2002b), to reinforce collaboration with several Eastern neighbors of the inflamed European Union, helped by the other members of the EU, demonstrate that new states can efficiently take part to flourishing program, or at slightly be significant partners of doing well initiatives offered together with some old states members. As consequence, the succession of the new states from East-Central Europe has not been as â€Å"detrimental† to the EU administration as it was at times recommended in Western Europe earlier than the enlargement. In conclusions, the effects of the Eastern enlargement on external and internal relations of the European Union have not been as â€Å"tragic† as it was occasionally feared prior to enlargement. The addition from 15 to 28 member nations, as well as the significant economic unevenness between old state and new state member have shaped some administration problems for the EU, but they have not busted it. The European Union ought to now focus on amplification of its present instruments and institutions. The new states have rapidly learned the Union’s regulations and procedures and to place political conciliation before majority of votes. Thus, the agreement code has retained its center value in the EU. The significance of the incorporation process is the vision of a new regional individuality based on resolution among the European countries. The Eastern enlargement has opened the way to a real unification of the continent. After the occurrence of two World Wars on its region, it behooves Europe not to lose this opportunity. Thus, harmony between the old states and new members remains the subject to a flourishing future of the society. References Baun, Michael 2004: â€Å"Intergovernmental Politics†. In: Nugent, Neill (ed.) European Union Enlargement. Palgrave Macmillan, pp. 132-145. Blockmans, Steven/Prechal, Sacha (eds.) 2008: Reconciling the Deepening and Widening of the European Union. The Hague: T.M.C Asser Press Dehousse, Renaud/Deloche-Gaudez, Florence/Duhamel, Olivier (eds.) 2006: Élargissement. Common l’Europes’adapte. Paris: Centre d’à ©tudes europà ©ennes, Presses Sciences Po. Dirk Leuffen 2010. â€Å"Breaking the Camel’s Back? Eastern Enlargement and EU Governance.† ECPR Paper Number 853, Center for Comparative and International Studies, ETH Zurich, p. 6 Faber, Anne 2009: â€Å"Eastern Enlargement in Perspective: A Comparative View on EC/EU Enlargements†. In: Loth, Wilfried (ed.): Experiencing Europe. 50 Years of European Construction 1957-2007. Baden-Baden: Nomos, pp. 305-325. Falkner, Gerda 1996: â€Å"Enlarging the European Union†. In: Richardson, Jeremy J. (ed.) European Union. Power and policy-making. London/New York: Routledge, pp. 233-246 Guà ©rot, Ulrike 2005: †Consequences and Strategic Impact of Enlargement on the (Old) EU†. In: Brimmer, Esther/ Frà ¶hlich, Stefan (eds.): The Strategic Implications of European Union Enlargement. Johns Hopkins University: Centre for Transatlantic Relations, pp. 53-72. Hagemann, Sara/De Clerck-Sachsse, Julia 2007: Decision-Making in the Council of Ministers: Evaluating the Facts. CEPS Policy brief No. 119, January 2007, available at http://www.ceps.be. Lindner, Johannes 2003: â€Å"Institutional stability and change: two sides of the same coin†. Journal of European Public Policy 10:6, December 2003, pp. 912-935. Miles, Lee 2004: â€Å"Theoretical Considerations†. In: Nugent, Neill (ed.) European Union Enlargement. Palgrave Macmillan, pp. 253-265. Nugent, Neill (ed.) 2004: European Union Enlargement. Palgrave Macmillan. Nugent, Neill 2004a: â€Å"Previous Enlargement Rounds.† In: Nugent, Neill (ed.): European Union Enlargement. PalgraveMacmillan, pp. 22-33. Nugent, Neill 2004b: â€Å"Distinctive and Recurrent Features of Enlargement Rounds.† In: Nugent, Neill (ed.): European Union Enlargement. Palgrave Macmillan, pp. 56-69. Preston, Christopher 1997: Enlargement and Integration in the European Union. London/New York: Routledg Schmitter 2004: â€Å"Neo-Neofunctionalism†. In: Wiener, Antje/Diez, Thomas (eds.): European Integration Theory. Oxford: Oxford University Press, pp. 45-74. Stacey, Jeffrey/Rittberger, Berthold 2003: â€Å"Dynamics of formal and informal institutional change in the EU†. Journal of European Public Policy 10:6, December 2003, pp. 858-883. Steunenberg, Bernard (ed.) 2002: Widening the European Union. The politics of institutional change and reform. London/New York: Routledge. Steunenberg, Bernard 2002a: â€Å"Enlargement and reform in the European Union†. In: Steunenberg, Bernard (ed.): Widening the European Union. The politics of institutional change and reform. London/New York: Routledge, pp. 3-20. Steunenberg, Bernard 2002b: â€Å"An even wider Union. The effects of enlargement on EU decision-making†. In: Steunenberg, Bernard (ed.): Widening the European Union. The politics of institutional change and reform. London/ New York: Routledge, pp. 97-118.

A Study On Immigration Case Study Social Policy Essay

A Study On Immigration Case Study Social Policy Essay Using the very large variation in the inflow of immigration across the states of US and other countries states we examine the force of immigration employment, average working hours, money growth and one most important, the total factors efficiency. At the same time we find striking proof that the total factors of increased productivity. These are some of the very robust reasons of controlling for many other determinants of efficiency that may vary with topography such as RD expenditure, computer implementation, international rivalry in the form of sectors composition and exports. Thus many results suggest that immigration promotes efficiency task specialization and at the same time promotes the adaption of amateurish-based technology as the technological change would forecast. (Peri, November 2009) In what ways can immigration help economy? Immigration does not spoil the Economy nor does any Government lose their money because of immigrants. After all the people of the country makes more money. The common idea that money passes from government to company to clients and back again, if a minor amount of money is moved from manufactures to clients then at the same time a larger amount of money is retained. Immigrants, if accepted as general public, are entitled to benefits. Thus these people are draining over 2 billion dollars from benefits each year. However, once these people get familiar to our ways of living, then they can become very helpful members of society. A basic reality about our economy is that as long as we infuse more supplies and services than we have, the more number of jobs which is nearly limitless. In reality, when the number of workers increases, the more the number of jobs we create. Thus the total number of services and the mass of the labor power has been tracked each other then reasonably closely for 50 years despite dramatic changes in immigration flows. Its a recognized fact that many of the jobs that immigrants are taking are that jobs that Americans are not taking. But we check immigrants from taking these vacant jobs, American producers and clients suffer the cost. (Powell, Friday, January 13, 2006) Do you think economies should welcome immigration? Yes definitely the economies should welcome immigration. An instance is the recent policies adopted by countries like Spain. Suppose that if a western nation were to loosen its borders, thus leading to an overflow of immigrants into that nation, the predicted outcome would be unemployment, rise in crime, social unrest, but Spain Disagrees on these predictions. Over a period of the last ten years, Spain has taken in millions of immigrants from diverse backgrounds, but still it has emerged as the best economy in Europe. The growth average has been 3.2% since the last half decade. Spain is responsible for the creation of 50% of the new jobs in the Euro zone and even unemployment has fallen down. All these factors have been credited to immigration. Immigrants start out by taking the jobs that are unwanted by the local population. The taxes that they pay contribute for more than the public amenities they use. Not only Spain, many other countries have adopted a pro-immigration stand and the success they have achieved is a testimony to the fact that economies should welcome immigration. Immigration also leads to intermixing of societies, thus providing a gateway to the concept of â€Å"ONE WORLD†. (spain:immigrants Welcome, 2007) References Giovanni Peri. (2009). Theeffect of Immigration on productivity: Evidence from US States. NBER Working Paper(15507) Benjamin Powell. (2006).Get this straight: Immigration helps economy. Retrieved March 13, 2010. http://sacramento.bizjournals.com/sacramento/stories/2006/01/16/editorial3.html Carol Matlack, Joan Tarzian. (2002). Spain: Immigrants Welcome Retrieved March 13, 2010.http://www.businessweek.com/magazine/content/07_21/b4035066.htm

Thoughts on a Possible Rational Reconstruction of the Method of Essay

Thoughts on a Possible Rational Reconstruction of the Method of "Rational Reconstruction" ABSTRACT: Rational reconstructions standardly operate so as to transform a given problematic philosophical scientific account-particularly of a terminological, methodological or theoretical entity-into a similar, but more precise, consistent interpretation. This method occupies a central position in the practice of analytic philosophy. Nevertheless, we encounter-even if only in a very few specific publications-a vague image of it. This is due on the one hand to the problem of the intentions of application, i.e., of the normativity of rational reconstruction (descriptive/prescriptive-ambivalence). It is also due on the other hand to the problem of the significance of the method in the field of history of philosophy (systematic/historical-dichotomy). The varied usage within analytic philosophy, as well as the increasingly inflationary and interfering usage outside, contribute to make rational reconstruction somehow appear a Proteus in contemporary philosophical methodology. This paper attempts to administer first aid and to close a bit of the theoretical gap and thus to reach a more exact image for the interests of analytic philosophy. Self-application of the method appears to be the right remedy. A graduating rational reconstruction of a standard concept of rational reconstruction will be suggested, differentiating the concept of rational reconstruction according to normativity, and explicating the method of rational reconstruction into two such variants. Introduction The method of rational reconstruction occupies a central position in the practice of Analytical Philosophy. Andreas Kamlah once has dealt with it in an article under ... ...85), pp.71-82 Poser, Hans [1971]: Philosophiegeschichte und rationale Rekonstruktion. Wert und Grenze einer Methode. In: Studia Leibnitiana 3 (1971), pp. 67-76 ________ [1980]: "Rekonstruktion, rationale." In: Speck, Josef (ed.): Handbuch wissenschaftstheoretischer Begriffe. Band. 3, Gttingen 1980, pp. 555-556 Stegmller, Wolfgang [1967]: Gedanken ber eine mgliche rationale Rekonstruktion von Kants Metaphysik der Erfahrung. Teil I. Kants Rtsel der Erfahrungserkenntnis. In: Ratio 9 (1967), pp. 1-30 ________ [1973]: Probleme und Resultate der Wissenschaftstheorie und Analytischen Philosophie. Band IV. Personelle und Statistische Wahrscheinlichkeit. Studienausgabe Teil A, Berlin 1973 ________ [1985]: Probleme und Resultate der Wissenschaftstheorie und Analytischen Philosophie. Band II. Theorie und Erfahrung. Studienausgabe Teil E, 2nd ed., Berlin 1985.

A Suitable Base Material for Composite Resin Restorations

Food and Chemical Toxicology 45 (2007) 1650–1661 www. elsevier. com/locate/foodchemtox A comparison of chemical, antioxidant and antimicrobial studies of cinnamon leaf and bark volatile oils, oleoresins and their constituents q Gurdip Singh b a,* , Sumitra Maurya a,1 , M. P. deLampasona b, Cesar A. N. Catalan b a Chemistry Department, DDU Gorakhpur University, Gorakhpur 273 009, India Instituto de Quimica Organica, Universidad Nacional de Tucuman, Ayacucho 471, S. M. de Tucuman 4000, Argentina Received 31 August 2005; accepted 22 February 2007Abstract The antioxidant, antifungal and antibacterial potentials of volatile oils and oleoresin of Cinnamomum zeylanicum Blume (leaf and bark) were investigated in the present study. The oleoresins have shown excellent activity for the inhibition of primary and secondary oxidation products in mustard oil added at the concentration of 0. 02% which were evaluated using peroxide, thiobarbituric acid, p-anisidine and carbonyl values. Moreove r, it was further supported by other complementary antioxidant assays such as ferric thiocyanate method in linoleic acid system, reducing power, chelating and scavenging e? cts on 1,1 0 -diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals. In antimicrobial investigations, using inverted petriplate and food poison techniques, the leaf and bark volatile oils has been found to be highly e? ective against all the tested fungi except Aspergillus ochraceus. However, leaf oleoresin has shown inhibition only for Penicillium citrinum whereas bark oleoresin has caused complete mycelial zone inhibition for Aspergillus ? avus and A. ochraceus along with Aspergillus niger, Aspergillus terreus, P. citrinum and Penicillium viridicatum at 6 lL. Using agar well di? sion method, leaf volatile oil and oleoresin have shown better results in comparison with bark volatile oil, oleoresin and commercial bactericide, i. e. , ampicillin. Gas chromatographic–mass spectroscopy studies on leaf volatil e oil and oleoresin resulted in the identi? cation of 19 and 25 components, which accounts for the 99. 4% and 97. 1%, respectively of the total amount and the major component was eugenol with 87. 3% and 87. 2%, respectively. The analysis of cinnamon bark volatile oil showed the presence of 13 components accounting for 100% of the total amount. E)-cinnamaldehyde was found as the major component along with d-cadinene (0. 9%), whereas its bark oleoresin showed the presence of 17 components accounting for 92. 3% of the total amount. The major components were (E)-cinnamaldehyde (49. 9%), along with several other components. O 2007 Elsevier Ltd. All rights reserved. Keywords: Cinnamomum zeylanicum Blume; Eugenol; Cinnamaldehyde; Antioxidant assay 1. Introduction Free radical reactions occur in human body and food systems. Free radicals, in the form of reactive oxygen and Part 57.Corresponding author. Tel. : +91 551 2200745 (R)/2202856 (O); fax: +91 551 2340459. E-mail address: [email  p rotected] com (G. Singh). 1 Present address: Agarkar Research Institute, Pune 411 004, India. * q nitrogen species, are an integral part of normal physiology. An over production of these reactive species can occur, due to oxidative stress brought about by the imbalance of bodily antioxidant defence system and free radical formation. These reactive species can react with biomolecules, causing cellular injury and death.This may lead to the development of chronic diseases such as cancers and those that involve the cardio- and cerebrovascular systems. The consumption of fruits and vegetables (Peschel et al. , 2006) containing antioxidants has been found to o? er protection 0278-6915/$ – see front matter O 2007 Elsevier Ltd. All rights reserved. doi:10. 1016/j. fct. 2007. 02. 031 G. Singh et al. / Food and Chemical Toxicology 45 (2007) 1650–1661 1651 against these diseases. Dietary antioxidants can augment cellular defences and help to prevent oxidative damage to cellular c omponents (Halliwell, 1989).Besides playing an important role in physiological systems, antioxidants have been used in food industry to prolong the shelf life of foods, especially those rich in polyunsaturated fats. These components in food are readily oxidized by molecular oxygen and are major cause of oxidative deterioration, nutritional losses, o? ?avour development and discoloration. The addition of synthetic antioxidants, such as propyl gallate, butylated hydroxylanisole (BHA), butylated hydroxyltoluene (BHT) and tertiary butylhydroquinone has been widely used industrially to control lipid oxidation in foods.However, the use of these synthetic antioxidants has been questioned due to their potential health risks and toxicity (Kahl and Kappus, 1993). The search for antioxidants from natural sources has received much attention and e? orts have been put in to identify compounds that can act as suitable antioxidants to replace synthetic ones. In addition, these naturally occurring a ntioxidants can be formulated as functional foods and nutraceuticals that can help to prevent oxidative damage from occurring in the body.Plants contain a variety of substances called ‘‘Phytochemicals’’ (Pratt, 1992), that owe to naturally occurring components present in plants (Caragay, 1992). The phytochemical preparations with dual functionalities in preventing lipid oxidation and antimicrobial properties have tremendous potential for extending shelf life of food products. Several research groups around the world have succeeded in ? nding and identifying natural antioxidants from herbs and spices using di? erent model systems.The antioxidant activity of Labiatae herbs such as rosemary, sage, summer savory and borage are also well documented (Bandoniene et al. , 2002; Djarmati et al. , 1991; Ho et al. , 2000; Aruoma et al. , 1996; Cuvelier et al. , 1994; Wong et al. , 1995; Chang et al. , 1997; Madsen et al. , 1996; Gordon and Weng, 1992; Takacsova et al. , 1995). However, the aromatic spicy and medicinal plants from Laureceae family are less extensively studied. Cinnamon (Cinnamomum zeylanicum Blume, syn C. verum, family Laureceae) is a widely used spice and have many applications in perfumery, ? voring and pharmaceutical industries. Although, the chemical constituents of leaf and bark essential oils of cinnamon have been studied (Raina et al. , 2001; ? Simic et al. , 2004; Jayaprakash et al. , 1997), the potential antioxidant properties have yet not been studied and it seems that investigation on oleoresins are scarce. Hence, in the present work, attempt has been made to explore the possible antioxidant and antimicrobial properties by di? erent methods which can give more comprehensive information especially when the e? ectiveness of multi component natural oleoresins is investigated.The objective of present investigation is to compare the chemical composition of leaf and bark essential oils and oleoresins as well as demonstrate t he possibility of protecting the stored food materials against micro-organism and antioxidative behaviour on mustard oil using as additive by various methods. 2. Materials and methods 2. 1. Chemicals Thiobarbituric acid, pure components eugenol and cinnamaldehyde were received form Merck, Germany. Diphenylpicrylhydrazyl (DPPH), carbendazim were procured from Sigma (Sigma–Aldrich GmbH, Sternheim, Germany) and linoleic acid from Across (New Jersey, USA).BHT, BHA, and 2,4-dinitrophenylhydrazine were purchased from s. d ? ne-chem Ltd, Mumbai, India. Ampicillin was purchased from Ranbaxy Fine chemicals Ltd. , New Delhi, India. Crude mustard oil was purchased from local oil mill, Gorakhpur, India. All solvents used were of analytical grade. 2. 2. Sample extraction Cinnamon leaves and barks were purchased from local market of Gorakhpur, Uttar Pradesh, during January 2004 and voucher specimens were kept at the Herbarium of the Science faculty, DDU Gorakhpur University, Gorakhpur.Cinn amon leaves (250 g) and barks (50 mesh particle size) were hydrodistilled using Clevenger’s apparatus to yield essential oils (3. 1% and 2. 5%, respectively). Oleoresins were obtained by extracting 25 g of powdered spice with 250 mL of acetone for 2 h in a Soxhlet extractor. The solvent was evaporated by placing the sample in a vacuum drier under reduced pressure. The viscous oleoresins for leaves and barks, with yield 6. 9% and 9. 7%, respectively, were obtained. Both essential oils and oleoresins were stored in cold condition and until further use. 2. 3. Chemical characterization 2. . 1. Gas chromatography (GC) A Hewlett Packard 6890 (Analytical Technologies SA, Buenos Aires, Argentina) gas chromatograph equipped with column HP-5 (5% phenyl methylsiloxane, length 30 m  · inner diameter 0. 25 mm  · ? lm thickness 0. 25 lm) was used for the analysis whose injector and detector temperatures were maintained at 240 and 250  °C, respectively. The amount of the samples injec ted was 0. 1 lL in split mode (80:1). Carrier gas used was helium with a ? ow rate of 1. 0 mL minA1. The oven temperature for essential oils were programmed linearly as follows: 60  °C (1 min), 60– 185  °C (1.  °C minA1), 185  °C (1 min), 185–275  °C (9  °C minA1 ), 275  °C (5 min) whereas for oleoresins it was as follows: 70  °C (1 min), 70–170  °C (1. 5  °C minA1), 170  °C (1 min), 170–180  °C (9  °C minA1), 280  °C (5 min). 2. 3. 2. Gas chromatography–mass spectrometry (GC–MS) Analysis of volatile oils and oleoresins were run on a Hewlett Packard (6890) GC–MS system (Analytical Technologies SA, Buenos Aires, Argentina) coupled to a quadrupole mass spectrometer (model HP 5973) with a capillary column of HP-5MS (5% phenyl methylsiloxane, length = 30 m, inner diameter = 0. 25 mm and ? lm thickness = 0. 5 lm). The injector, GC–MS interface, ion source and selective mass detector temperatures were main tained at 280, 280, 230 and 150  °C respectively. The oven temperature programmed for the volatile oils were same as provided for GC whereas for oleoresins, it was programmed linearly as follows: 60– 185  °C (1. 5  °C minA1), 185  °C (1 min), 185–275  °C (9  °C minA1), 275  °C (2 min). The extract was held at 70  °C (5 min), 70–220  °C (3  °C minA1), 220–280  °C (5  °C minA1) and held at 280  °C for 5 min. 2. 3. 3. Components identi? cation The components of essential oil and oleoresins were identi? d on the basis of comparison of their retention indices and mass spectra with published data (Adams, 2001; Massda, 1976) and computer matching with WILEY 275 and National Institute of Standards and Technology (NIST 3. 0) libraries provided with computer controlling the GC–MS system. The results were also con? rmed by the comparison of the compounds elution order with their relative retention indices on non-polar phase 1652 G. S ingh et al. / Food and Chemical Toxicology 45 (2007) 1650–1661 2. 4. 2. DPPH and hydroxyl radical scavenging e? ects The DPPH assay was carried out as described by Brand-Williams and his co-workers (1995). , 10, 15, 20, 25 lL of the sample were added to 5 mL of 0. 004% methanol solution of DPPH. After a 30 min incubation period at room temperature, the absorbance was read against a blank at 515 nm. The assay was carried out in triplicate and analyses of all samples were run in duplicate and results are averaged. This test was adopted from a method described by Halliwell et al. (1987). Solutions of the reagents were always prepared freshly. The reaction mixture contained in a ? nal volume of 1. 0 mL, 100 lL of 2-deoxy-2ribose (28 mM in KH2PO4–K2HPO4 bu? er, pH 7. ), 500 lL of various concentrations of the tested oils or the pure compounds in bu? er, 200 lL of 1. 04 mM EDTA and 200 lM FeCl3 (1:1 v/v), 100 lL of 1. 0 mM H2O2 and 100 lL of 1. 0 mM ascorbic acid. Test sampl es were kept at 37  °C for 1 h. The free radical damage imposed on the substrate, deoxyribose, was measured using the thiobarbituric acid test (Ohkawa et al. , 1979; Shimada et al. , 1992). 1. 0 mL of TBA (1%), and 1. 0 mL tricholoroacetic acid (2. 8%) were added to the test tubes and were incubated at 100  °C for 20 min. After cooling, absorbance was measured at 532 nm against a blank containing deoxyribose and bu? r. Reactions were carried out in triplicate. Inhibition (I) of deoxyribose degradation in percent was calculated in the following way: I? %? ? 100X ? A0 A A1 =A0 ? where A0 is the absorbance of the control reaction, and A1 is the absorbance of the test compound. 2. 4. 3. Chelating e? ect and reducing power Chelating e? ect was determined according to the method of Shimada et al. (1992). To 2 mL of the mixture, consisting of 30 mM hexamine, 30 mM potassium chloride and 9 mM ferrous sulphate were added to 5, 10, 15, 20, 25 lL of essential oil or oleoresin in methanol ( 5 mL) and 200 lL of 1 mM tetramethyl murexide.After 3 min at room temperature, the absorbance of the mixture was determined at 485 nm. A lower absorbance indicates a higher chelating power. EDTA was used as a positive control. The reducing power was carried out as described before (Oyaizu, 1986). Various amount (5, 10,15, 20 lL) of essential oil or oleoresin (dissolved in 2. 5 mL of methanol) mixed with 2. 5 mL of 200 mM phosphate bu? er (pH = 6. 6) and 2. 5 mL of 1% potassium ferricyanide, and the mixture was incubated at 50  °C for 20 min. After adding 2. 5 mL of 10% trichloroacetic acid, the mixture was centrifuged at 200 g for 10 min in Sigma 3K30 model centrifuger.The organic layer (5 mL) was mixed with 5 mL of deionised water and 1 mL of 0. 1% ferric chloride and the absorbance read at 700 nm in a UV–visible spectrophotometer. reported in the literature (Adams, 2001). The retention indices were calculated for all volatile constituents using a homologous series of n-al kanes C8–C16. 2. 3. 4. Antioxidative assays in mustard oil Oxidative deterioration was monitored under modi? ed Shaal Oven test (Economou et al. , 1991). Leaf and bark essential oils and oleoresins along with synthetic antioxidants and major components were added individually to unre? ned mustard oil at levels of 0. 2% (v/v). The initial PV value of oil is 1. 7 meq of O2/kg. Oxidative deterioration was periodically assessed by measuring the antioxidant parameters such as peroxide (PV), thiobarbituric acid (TBA), p-anisidine (p-An) and total carbonyl (TC) values. 2. 3. 5. PV and TBA values The rate of oil oxidation was monitored by the increase of peroxide values. About 3 g of each oil sample was weighed and subjected to iodimetric determination (AOCS, 1990). TBA values were evaluated according to the methods previously stated by some authors (Sidwell et al. , 1954) with small changes. To 10 g of oil sample, 0. 7% aq. thiobarbituric acid (20 mL) and benzene (25 mL) solution we re added. This mixture was shaken continuously for 2 h using mechanical shaker. After 2 h, supernatant was taken and placed in boiling water-bath for 1 h. After cooling, absorbance of supernatant was measured at 540 nm with Hitachi-U-2000 spectrophotometer. 2. 3. 6. p-Anisidine value The test was performed according to the methods (AOCS, 1998,) previously stated by earlier workers (Ottolenghi, 1959; Kikuzaki and Nakatani, 1993). In a 50 mL volumetric ? ask, 0. 6 g of oil sample was taken and volume was made using isoctane solution.From this solution, 5 mL was treated with 1 mL of 0. 25% of p-anisidine reagent and kept in dark for 10 min and absorbance was measured at 350 nm using a UV–VIS spectrophotometer. 2. 3. 7. Total carbonyl value Carbonyl value was evaluated according to the methods as reported earlier (Frankel, 1998). About 4 g of sample was taken in a 50 mL volumetric ? ask and the volume was made up using carbonyl free benzene. Out of this, 5 mL was pippeted out and mixed with 3 mL of 4. 3% trichloroacetic acid and 5 mL of 2,4-dinitrophenyl hydrazine (0. 05% in benzene) in 50 mL volumetric ? asks.The mixture was incubated at 60  °C for half an hour to convert free carbonyls into hydrazones. After cooling, 10 mL of KOH solution (4% in ethanol) was added and the volume was made with ethanol. After 10 min, absorbance was measured at 480 nm using UV–VIS spectrophotometer. Blank was prepared in the same manner substituting 5 mL of benzene instead of sample. A standard curve was drawn using valeraldehyde (50–250 lg) in 5 mL of benzene instead of sample. The total carbonyl was calculated with the help of the standard curve and expressed as mg of valeraldehyde per 100 g of sample. 2. 5. Antimicrobial activity 2. 5. . Antifungal investigations In order to determine the antifungal e? cacy of the volatile oil and its oleoresin, the pathogenic fungus Aspergillus niger, Aspergillus ? avus, Aspergillus ochraceus, Aspergillus terreus, Fusariu m moniliforme, Fusarium graminearum, Penicillium citrinum and Penicillium viridicatum were undertaken. These fungi were isolated from food materials such as onion, vegetable waste, wheat straw, fruits of Musa species, sweet potato, decaying vegetation and vegetable, respectively and were procured from Microbial Type Culture Collection (MTCC), Institute of Microbial Technology, Chandigarh, India.The MTCC code No. of these strains are 2479, 1884, 1810, 3374, 1893, 2088, 2553 and 2007, respectively. Cultures of each of the fungi were maintained on Czapek (DOX) agar media with adjusting pH 6. 0–6. 5 and slants were stored at 4  °C. The antifungal activity of the volatile oil and oleoresin against fungi were undertaken using inverted petriplate (Ramdas et al. , 1998) and poison food techniques (Amvam Zolla et al. , 1998). In inverted petriplate method, the required dose (2, 4 and 6 lL) of undiluted sample were soaked on a small piece (diameter 12 mm) of Whatmann No. 1 ? ter pape r and it was kept on the lid of petriplate which is in inverted position whereas in poison food 2. 4. Complementary antioxidant assays 2. 4. 1. Antioxidant activity in linoleic acid system Antioxidant activity was carried out using the method proposed by Osawa and Namaki (1983) with small changes. Samples (1 mL) in ethanol were mixed with 2. 5% linoleic acid in ethanol (4. 1 mL), 0. 05 M phosphate bu? er (pH = 7, 8 mL) and distilled water (3. 9 mL) and kept in screw cap containers under dark condition at 40  °C. This solution (0. 1 mL) was added to the solution of 9. 7 mL of 75% ethanol and 0. mL of 30% ammonium thiocyanate. After 3 min, 0. 1 mL of 0. 02 M ferrous chloride in 3. 5% hydrochloric acid was added to the reaction mixture, the absorbance of red color was measured at 500 nm in the spectrophotometer, for every two days. The control and standards were subjected to the same procedure except for the control, where there was no addition of sample and for the standard 1 mL of sample was replaced with 1 mg of BHA and BHT. G. Singh et al. / Food and Chemical Toxicology 45 (2007) 1650–1661 technique, the required dose (2, 4 and 6 lL) of the undiluted sample were mixed with the 20 mL of culture medium.Each test was replicated for three times and fungi toxicity was measured after 6 days in terms of percent mycelial zone inhibition. 2. 5. 2. Antibacterial investigations Six pathogenic bacteria Bacillus cereus (430), Bacillus subtilis (1790), Staphylococcus aureus (3103) (gram-positive), Escherichia coli (1672), Salmonella typhi (733), Pseudomonas aeruginosa (1942) (gram-negative) were selected for present study. All the bacterial strains were procured from Microbial Type Culture Collection (MTCC), Institute of Microbial Technology, Chandigarh, India. They were sub cultured on nutrient agar broth (Hi-media) and stored at 4  °C.Active cultures for experiments were prepared by transferring one loopful of cells from stock cultures to ? ask of nutrient aga r broth, which were incubated without agitation for 24 h at 37  °C. In order to determine the antibacterial activity of the essential oils and oleoresins, agar well di? usion method was followed. 0. 1 mL of 101 time diluted bacterial strain in ringers solution were ? ood inoculated on to the surface of well settled sterilized culture medium. The wells (10 mm diameter) were cut from agar, and 0. 2 mL of sample (2, 4 and 6 lL of essential oil or oleoresin diluted in 1 mL of DMSO) was delivered into them.For standard, 0. 2 mL of aqueous solution of ampicillin (1 mg mLA1) was used. After incubation for 24 h at 37  °C, all plates were examined for any zones of growth inhibition according to method developed by Davidson and Parish (1989). All the plates were replicated twice and the results were averaged. 2. 5. 3. Statistical analysis For the oil or oleoresin, three samples were prepared for each experiment. The data were presented as mean  ± standard deviation of three determinatio ns (data were not shown). The quantitative data of major components of oil and oleoresin were statistically examined by analysis of variance (Sokal, 1973) and signi? ant di? erences among several groups of data were examined by Ducan’s multiple range test. A probability value of p < 0. 05 was considered signi? cant. Table 1 Chemical composition of cinnamon leaf volatile oil and oleoresin Compound Volatile oil MS % a-Thujene a -Pinene b-Pinene Myrcene a-Phellandrene p-Mentha-1(7),8-diene p-Cymene 1,8-Cineole Terpinolene a-Terpineol a-Cubebene Eugenol b-Caryophyllene Aromadendrene a-Amorphene Germacrene-D Bicyclogermacrene d-Cadinene Spathulenol Sabinene c-Terpinene Terpinen-4-ol d-Elemene Viridi? orol Methoxy-eugenol Isospathulenol Neophytadiene Docosane Nonacosane Vitamin-E Total 0. 1 0. tr tr 1. 9 tr 0. 7 0. 7 tr tr tr 87. 3 1. 9 1. 1 tr 0. 6 3. 6 0. 4 0. 5 – – – – – – – – – – – 99. 4% a 1653 Oleore sin KI 931 941 980 993 1007 1011 1026 1033 1088 1191 1350 1358 1420 1441 1490 1490 1496 1527 1576 – – – – – – – – – – – MSa % – – – – 0. 3 – tr – – tr – 87. 2 1. 4 0. 8 0. 4 0. 2 1. 7 0. 6 1. 7 tr tr tr 1. 0 0. 3 0. 1 0. 3 0. 3 0. 1 0. 1 0. 2 97. 1% KI – – – – 1007 – 1026 – – 1191 – 1358 1420 1441 1490 1490 1496 1527 1576 975 1064 1177 1340 1594 – – – – – – 3. Results and discussion 3. 1. Chemical analysis GC and GC–MS analysis of cinnamon leaf volatile oil showed the presence of 19 components accounting for 99. % of the total amount (Table 1). The major component was eugenol (87. 3%) followed by bicyclogermacrene (3. 6%), a-phellanderene (1. 9%), b-carryophyllene (1. 9%), aromadendrene (1. 1%), p-cymene (0. 7%) and 1,8-cineole (0. 7%). Moreover, it s oleoresin showed the presence of 25 components accounting for 97. 1% of the total amount (Table 1). The major components accounting were eugenol (87. 2%), spathulenol (1. 7%), bicyclogermacrene (1. 7%), b-caryophyllene (1. 4%) and d-elemene (1. 0%). The analysis of cinnamon bark volatile oil showed the presence of 13 components accounting for 100% of the total amount (Table 2). E)-cinnamaldehyde was found as the major component along with d-cadinene (0. 9%), a-copaene (0. 8%) and a-amorphene (0. 5%), whereas its bark oleoresin showed the presence of 17 components accounting for 92. 3% of the total amount (Table 2). The major components were (E)-cinnamaldehyde (49. 9%), coumarin (16. 6%), d-cadinene (7. 8%), a-copaene (4. 6%), (Z)-cinnamaldehyde (1. 5%), ortho-methoxy cinnamaldehyde (1. 5%) and b-bisabolene (1. 4%) along with several other compo- Percentages are the mean of three runs and were obtained from electronic integration measurements using selective mass detector tr < 0. 1 . a nents. Recently, Raina et al. (2001) reported eugenol (76. 6%), linalool (8. 5%) and pipertone (3. 31%) as major components from its leaf oil grown in little Andman whereas the steam distilled volatile oil of cinnamon fruit ? grown at Karnataka and Kerala consists (Simic et al. , 2004; Jayaprakash et al. , 1997) of hydrocarbons (32. 8% and 20. 8%) and oxygenated compounds (63. 7% and 73. 4%) and trans-cinnamyl acetate and b-caryophyllene were found to be major component. 3. 2. Antioxidative assays in mustard oil The changes of PV in mustard oil of all investigated samples are presented in Fig. 1.The rate of oxidative reactions in mustard oil with additives was almost similar to that of the blank sample. The stability of the mustard oil samples to the formation of peroxides can be ranked in the following descending order: Leaf oleoresin > BHT > PG % eugenol > Bark oleoresin % BHA > Leafoil > cinnamaldehyde > bark oil 1654 G. Singh et al. / Food and Chemical Toxicology 45 (2007) 1 650–1661 Table 2 Chemical composition of cinnamon bark volatile oil and extract Compound Volatile oil MS % a-Pinene Camphene Sabinene b-Pinene Limonene 1,8-Cineole Camphor Z-cinnamaldhyde E-cinnamaldhyde a-Copaene a-Amorphene -Cadinene Terpinen-4-ol b-Caryophyllene Coumarin a-Muurolene b-Bisabolene Cadina-1(2), 4-diene Ortho-methoxy cinnamadehyde Cubenol 1-Heptadecene 1-Nonadecene Tetracosane Octacosane Nonacosane Total a a Oleoresin KI 941 953 975 980 1031 1035 1144 1225 1279 1379 1490 1527 – – – – – – – – – – – – – MSa % – – – – – – – 1. 5 50. 0 4. 6 – 7. 8 0. 1 1. 0 16. 6 4. 4 1. 4 1. 8 1. 5 0. 5 0. 2 0. 4 0. 1 0. 1 0. 2 92. 3% KI – – – – – – – 1225 1279 1379 – 1527 1177 1420 1436 1500 1506 1530 1532 – – – – – – tr tr tr tr tr tr tr tr 97. 7 0. 8 0. 5 0. 9 – – – – – – – – – – – – – 100% ays. The e? ects of volatile oils and oleoresins on malonaldehyde formation for mustard oil in terms of incubation time versus TBA value at 60  °C are shown in Fig. 2. The malondehyde formation of all the additives increases with storage time. The oil showed a moderate inhibition at 0. 02% concentration, and was comparable to BHA and PG but much lower than BHT. These results were well correlated with p-anisidine and total carbonyl values (Fig. 4). However, the sequence is slightly di? erent as compared with the one obtained during measurements of peroxide values.For instance, bark oleoresin had a little greater activity for preventing the formation of secondary oxidation products than primary ones. On contrary, volatile oils were slightly less e? ective in preventing the formation of secondary oxidation products than primary ones. From the above results, it should be said that the formation of the primary oxidation species, peroxides, were also quite similar with the secondary oxidation products, and the changes of both oxidation characteristics are in a good correlation. Hence, the inhibition activity of leaf and bark oleoresins were excellent among all the additives and there was a signi? ant di? erence between the blank and antioxidants at the P < 0. 05 level. 3. 3. Antioxidant activity in linoleic acid system To evaluate the antioxidant potential of volatile oils and oleoresins of leaf and bark, their lipid inhibitory activities were compared with selected antioxidants and their major components by using ferric thiocyanate method of measuring the amounts of peroxides formed in emulsion during incubation. High absorbance is an indication of a high concentration of formed peroxides. The absorbance values of volatile oils and oleoresins of cinnamon along with synthetic antioxidants are shown in Fig. . The absorba nce Percentages are the mean of three runs and were obtained from electronic integration measurements using selective mass detector tr < 0. 01. Simultaneously with the measurements of peroxide value, the changes the secondary oxidation products such as malonaldehyde and 2-alkenals, which are measured by thiobarbituric (Fig. 2), p-anisidine (Fig. 3) and total carbonyl values (Fig. 4), were also determined after every 7 120 Control BHT C. L. Oil C. L. Oleoresin eugenol BHA PG C. B. Oil C. B. Oleoresin E-cinnamaldehyde 100 Peroxide value (meq/kg) 80 60 40 20 0 0 7 14 21 28Incubation time (days) Fig. 1. Inhibitory e? ect of volatile oil and oleoresin of cinnamon leaf and bark on the primary oxidation of mustard oil measured using peroxide value method. G. Singh et al. / Food and Chemical Toxicology 45 (2007) 1650–1661 1655 6 5 Control BHT Leaf oil Leaf oleoresin Eugenol BHA PG Bark oil Bark oleoresin E-cinnamaldehyde TBA value (meq/g) 4 3 2 1 0 0 7 14 21 28 Incubation time (days) Fig. 2. Inhibitory e? ect of volatile oil and oleoresin of cinnamon leaf and bark on the malonaldehyde formation in mustard oil measured using TBA value method. 7 6 Control BHT C. L. Oil C. L.Oleoresin eugenol BHA PG C. B. Oil C. B. Oleoresin E-cinnamaldehyde p-anisidine value 5 4 3 2 1 0 0 7 14 21 28 Incubation time (days) Fig. 3. Inhibitory e? ect of volatile oil and oleoresin of cinnamon leaf and bark on the formation of 2-alkenals in mustard oil measured using p-anisidine method. 16 14 Carbonyl value (mg) 12 10 8 6 4 2 0 7 Control BHT C. L. Oil C. L. Oleoresin Eugenol BHA PG C. B. Oil C. B. Oleoresin E-cinnamaldehyde 14 21 28 Incubation time (days) Fig. 4. Inhibitory e? ect volatile oil and oleoresin of cinnamon leaf and bark on the total carbonyls present in mustard oil. 1656 G. Singh et al. Food and Chemical Toxicology 45 (2007) 1650–1661 1. 9 1. 7 Absorbance at 500 nm 1. 5 1. 3 1. 1 0. 9 0. 7 0. 5 0 Control BHT Leaf oleoresin Bark oleoresin Cinnamaldehyde BHA Leaf oil bark oil eugenol 25 50 75 100 125 150 175 200 Incubation time (h) Fig. 5. Inhibitory e? ect of volatile oil and oleoresin of cinnamon leaf and bark on the primary oxidation of linoleic acid system measured using ferric thiocyanate method. of linoleic acid emulsion without additive increased rapidly, and there was a signi? cant di? erence between blank and antioxidants at the P < 0. 05 level. As can be seen in this ? , bark oleoresin was most e? ective among all the additives followed by leaf oleoresin. However, there are no signi? cant (p < 0. 05%) di? erences between antioxidative activities of oleoresins, oils, BHA, BHT and PG. 3. 4. DPPH and hydroxyl radical scavenging e? ects Table 6 shows the DPPH and hydroxyl radical scavenging activity of leaf and bark volatile oils and oleoresins with various concentrations. As positive control, BHA and BHT were also examined. Bark oleoresin showed the best result through all concentrations for DPPH assay. The volatile oils have shown almos t equal and moderate radical scavenging activity.At a concentration of 5 lL, signi? cant di? erences in DPPH scavenging activities was observed between BHA (78. 4%), BHT (81. 2%) and oleoresins of both leaf (51. 3%) and bark (75. 6%). However, as concentration increased, the di? erences in scavenging activities between BHA, BHT and oleoresins become less signi? cant. For hydroxyl radical scavenging test AOH radicals were generated by reaction of ferric-EDTA together with H2O2 and ascorbic acid to attack the substrate deoxyribose. The resulting products of the radical attack form a pink chromogen when heated with TBA in acid solution (Ohkawa et al. , 1979; Shimada et al. 1992). When the oils or oleoresins were incubated with this reaction mixture they were able to interfere with free radical reaction and could prevent damage to the sugar. The results are shown in Table 6. At 5 lL, scavenging e? ects on hydroxyl radicals were 31. 2%, 51. 2%, 43. 6% and 57. 6% for leaf and bark volatil e oils and oleoresin. However, at 25 lL BHA and BHT exhibited scavenging activities of 84. 9% and 83. 2%, respectively. There was a little change in the order of DPPH and hydroxyl radical scavenging activity of leaf oleoresin (86. 1%), bark volatile oil (79. 6%) and bark oleoresin (78. 6%).A close to linear correlation between radical scavenging activity and concentration of polyphenolic compounds in various vegetable and fruits have been reported (Pyo et al. , 2004; Robards et al. , 1999). These reports indicated that the radical scavenging activity of oleoresins might be mostly a? ected by position of the phenolic hydroxyl group which is present in eugenol. Yepez et al. (2001) used eugenol as standard which removed 95% of the initial DPPH free radical. 3. 5. Chelating e? ect and reducing power Chelating e? ects of the leaf and bark oleoresins on ferrous ions increased from 20. 5% at 5 lL to 24. % at 10 lL and maintained a plateau of 28. 2–35. 5% at 15– 25lL (Fig. 6). The bark oleoresin showed a better chelating e? ect than those leaf oleoresin and both volatile oils. In addition, chelating e? ects of oleoresins were relatively parallel and increased from 20. 5–23. 6% at 5 lL to 38. 5– 42% at 25 lL. However, at 5 lL, the chelating ability of EDTA was 90. 4%. Apparently, the cinnamon leaf and bark oleoresins could chelate ferrous ions but were not as e? ective chelators as EDTA. Reducing powers of leaf and bark oleoresins of cinnamon were excellent and were in the range 56. 0–58. 4, comparable with that of BHA (63. ) and BHT (65. 2) at 5 lL (Fig. 7). However, at 25 lL, the reducing power of the leaf and bark oleoresins, BHA and BHT were comparable (78. 5–87. 9). The reducing powers of the oleoresins might be due to the hydrogen donating abilities (Shimada et al. , 1992). 3. 6. Antimicrobial studies The results of volatile oils and oleoresins of cinnamon leaf and bark by inverted petriplate and poison food tech- G. Sing h et al. / Food and Chemical Toxicology 45 (2007) 1650–1661 1657 100 90 Chelating effect (%) 80 70 60 50 40 30 20 10 0 0 EDTA Leaf oleoresin Bark oleoresin E-Cinnamaldehyde Leaf oil Bark oil Eugenol 10 15 20 25 30 Concentration ( L) Fig. 6. Chelating e? ect of volatile oil and oleoresin of cinnamon leaf and bark along with synthetic antioxidants. 100 Reducing power (%) 80 BHA Leaf oil Bark oil Eugenol BHT Leaf oleoresin Bark oleoresin Cinnamaldehyde 60 40 20 0 5 10 15 20 25 30 Concentration ( L) Fig. 7. Reducing power of volatile oil and oleoresin of cinnamon leaf and bark along with synthetic antioxidants. niques are reported in Tables 3 and 4, respectively. Using inverted petriplate method (Table 3), the leaf volatile oil was found to be 100% antifungal against all the tested fungi except A. chraceus and A. terreus at 6 lL. It was interesting to note that complete inhibition against A. ?avus was obtained only at 2 lL. However, leaf oleoresin has shown complete mycelial zone inhibition only for P. citrinum. More than 75% activity was obtained for P. veridicatum, F. moniliforme and A. ?avus. Bark volatile oil has shown complete inhibition against the fungi such as F. gramenearum, F. moniliforme, P. citrinum, P. viridicatum and A. terreus at 6 lL. Using poison food technique (Table 4), leaf volatile has caused complete inhibition against all the tested fungi except P. itrinum whereas oleoresin has caused complete inhibition only against P. citrinum. Bark volatile oil has shown complete inhibition against almost all the tested fungi except for A. ?avus, A. ochraceus whereas its oleoresin has caused complete inhibition for A. ?avus and A. ochraceus along with A. niger, A. terreus, P. citrinum and P. viridicatum at 6 lL. Using agar well di? usion method (Table 5), leaf volatile oil has shown better results in comparison with oleoresin and commercial bactericide, i. e. , ampicillin. Complete mycelial zone inhibition was obtained using leaf volatile oil again st P. eruginosa and B. cereus. However, it has moderate inhibitory e? ect on B. subtilis and S. aureus whereas its oleoresin has shown almost 100% activities against S. typhi and B. cereus. Bark volatile oil has been found to be better than bark oleoresin as it has caused more than 50% inhibition against all the tested fungi. There are several reports (Singh et al. , 1995; Hili et al. , 1997) stating that C. zeylanicum Blume exhibit antimicrobial activity. Their results demonstrate that the leaf oil completely inhibit the growth of E. coli, S. aureus and P. aeruginosa at the 1658 G. Singh et al. Food and Chemical Toxicology 45 (2007) 1650–1661 Table 3 Antifungal activity of volatile oils and oleoresins of cinnamon leaf and bark by inverted petriplate method Test Dose (lL) Percent mycelial inhibition zonea AN Leaf volatile oil 2 4 6 2 4 6 2 4 6 2 4 6 2 4 6 2 4 6 91. 5 100 100 25. 0 50. 0 58. 7 85. 3 93. 1 100 6. 3 38. 7 87. 2 62. 5 100 100 6. 3 35. 1 78. 3 AF 100 100 100 45. 6 76. 3 89. 3 100 100 100 6. 3 8. 8 13. 8 81. 2 100 100 65. 3 93. 2 100 AO 18. 7 56. 3 87. 5 46. 3 56. 3 68. 7 15. 6 52. 8 85. 3 12. 5 25. 0 37. 5 54. 3 78. 7 100 12. 5 25. 0 30. 8 FG 50. 0 52. 5 100 37. 5 50. 56. 3 36. 3 45. 8 95. 2 87. 5 87. 5 100 25. 0 50. 0 58. 7 75. 0 87. 5 100 FM 50. 0 52. 5 100 57. 5 80. 0 92. 5 31. 2 43. 2 83. 6 75. 0 87. 5 100 58. 6 79. 5 83. 3 58. 7 75. 3 83. 8 PC 37. 5 56. 3 100 67. 8 93. 3 100 25. 5 45. 8 86. 3 100 100 100 100 100 100 100 100 100 PV 37. 5 56. 3 100 38. 9 65. 5 87. 5 28. 5 47. 3 93. 7 100 100 100 76. 5 87. 5 100 85. 5 91. 5 100 AT 18. 7 36. 5 75. 0 46. 3 56. 3 68. 7 41. 3 53. 2 69. 1 37. 5 56. 3 100 87. 5 94. 1 100 56. 3 85. 6 100 Leaf oleoresin Eugenol Bark volatile oil Bark oleoresin E-cinnamaldehyde AN = Aspergillus niger; AF = Aspergillus ? vus; AO = Aspergillus ochraceus; FG = Fusarium graminearum; FM = Fusarium moniliforme; PC = Penicillium citrinum; PV = Penicillium viridicatum; AT = Aspergillus terreus. a Average of three replicate s. Table 4 Antifungal activity of volatile oils and oleoresins of cinnamon leaf and bark by food poisoned method Test Dose (ppm)a Percent mycelial inhibition zonea AN Leaf volatile oil 2 4 6 2 4 6 2 4 6 2 4 6 2 4 6 2 4 6 1000 2000 3000 100 100 100 62. 5 77. 5 87. 5 100 100 100 73. 5 100 100 48. 9 65. 3 83. 6 52. 3 68. 7 72. 3 78. 2 82. 2 96. 3 AF 31. 3 87. 5 100 18. 8 50. 0 100 15. 6 63. 2 95. 6 (–) 51. 3 87. 5 88. 7 91. 3 100 52. 87. 6 91. 2 85. 3 91. 2 96. 2 AO 50. 0 100 100 35. 0 82. 5 97. 5 45. 6 95. 6 100 75. 0 81. 2 100 100 100 100 100 100 100 84. 2 91. 2 98. 4 FG 75. 0 100 100 62. 5 77. 5 87. 5 63. 5 82. 1 93. 8 50. 0 75. 0 87. 5 65. 3 83. 2 100 47. 2 67. 8 85. 3 90. 2 96. 3 94. 5 FM 100 100 100 38. 7 46. 3 78. 7 45. 6 53. 6 78. 3 75. 0 83. 2 100 48. 7 56. 3 78. 7 63. 2 65. 8 87. 1 97. 2 100 100 PC 50. 0 75. 0 87. 5 35. 0 62. 5 97. 5 48. 6 73. 1 82. 6 43. 7 51. 3 65. 0 100 100 100 85. 2 89. 7 91. 2 100 100 100 PV 87. 5 100 100 50. 0 65. 5 70. 0 73. 2 85. 6 93. 6 50. 0 75. 0 87. 5 60. 0 85. 3 100 55. 3 63. 1 91. 2 100 100 100 AT 18. 7 50. 0 56. (–) 50. 0 100 15. 5 50. 0 75. 2 32. 5 45. 0 76. 3 35. 0 76. 2 83. 7 42. 3 45. 6 89. 3 98. 5 100 100 Leaf oleoresin Eugenol Bark volatile oil Bark oleoresin E-cinnamaldehyde Carbendazimb AN = Aspergillus niger; AF = Aspergillus ? avus; AO = Aspergillus ochraceus; FG = Fusarium graminearum; FM = Fusarium moniliforme; PC = Penicillium citrinum; PV = Penicillium viridicatum; AT = Aspergillus terreus. a Average of three replicates. b Aqueous solution was used. G. Singh et al. / Food and Chemical Toxicology 45 (2007) 1650–1661 Table 5 Antibacterial activity of volatile oils and oleoresins of cinnamon leaf and bark by agar well di? sion method Test Concentration (ppm) Inhibition zone (mm)a Gram (+) bacteria Bs Leaf volatile oil 1000 2000 3000 1000 2000 3000 1000 2000 3000 1000 2000 3000 1000 2000 3000 1000 2000 3000 1000 2000 3000 17. 1  ± 0. 4 20. 0  ± 0. 6 32. 6  ± 1. 2 14. 6  ± 1. 2 19. 0  ± 0. 2 25. 4  ± 0. 8 14. 3  ± 0. 6 17. 0  ± 0. 3 29. 6  ± 1. 2 14. 2  ± 0. 5 18. 3  ± 0. 3 26. 7  ± 0. 7 16. 2  ± 1. 3 20. 2  ± 1. 1 25. 3  ± 0. 3 12. 3  ± 0. 1 17. 3  ± 0. 5 23. 7  ± 0. 6 32. 5  ± 1. 2 34. 3  ± 0. 3 41. 2  ± 0. 2 Sa 26. 1  ± 1. 5 34. 9  ± 1. 3 48. 7  ± 0. 5 27. 1  ± 0. 1 38. 9  ± 0. 2 49. 3  ± 2. 2 23. 1  ± 1. 1 26. 9  ± 1. 3 38. 7  ± 0. 3 27. 0  ± 0. 9 44. 6  ± 0. 56. 7  ± 0. 1 23. 1  ± 0. 4 28. 7  ± 0. 2 33. 6  ± 0. 3 23. 0  ± 0. 7 41. 6  ± 0. 8 53. 7  ± 0. 1 29. 5  ± 0. 6 32. 6  ± 1. 6 37. 5  ± 0. 2 Bc 43. 3  ± 1. 7 58. 0  ± 0. 6 + 64. 5  ± 0. 6 80. 4  ± 1. 1 + 33. 3  ± 1. 5 56. 0  ± 0. 8 72. 3  ± 0. 2 41. 3  ± 1. 7 52. 6  ± 1. 2 56. 3  ± 0. 5 38. 6  ± 0. 2 41. 3  ± 0. 4 45. 6  ± 0. 7 31. 3  ± 1. 2 48. 6  ± 0. 2 52. 3  ± 0. 3 31. 4  ± 0. 2 34. 6  ± 0. 1 38. 2  ± 0. 3 Gram (A) bacteria Ec 13. 0  ± 0. 2 18. 2  ± 1. 1 25. 8  ± 0. 5 11. 4  ± 0. 6 13. 1  ± 0. 7 18. 5  ± 1. 1 11. 3  ± 0. 1 17. 2  ± 1. 6 21. 8  ± 0. 3 28. 1  ± 0. 2 33. 2  ± 1. 3 35. 1  ± 0. 3 33. 4  ± 0. 5 35. 4  ± 0. 3 37. 1  ± 0. 3 26. 1  ± 0. 5 33.  ± 1. 8 34. 1  ± 0. 2 33. 6  ± 0. 8 37. 8  ± 1. 4 39. 5  ± 0. 6 St 12. 5  ± 0. 8 14. 6  ± 1. 1 17. 9  ± 0. 2 53. 6  ± 1. 3 73. 8  ± 0. 5 78. 1  ± 0. 8 12. 5  ± 0. 8 14. 6  ± 1. 1 17. 9  ± 0. 2 20. 6  ± 1. 8 32. 7  ± 2. 0 41. 3  ± 0. 3 17. 2  ± 0. 1 18. 6  ± 0. 7 19. 3  ± 0. 5 18. 6  ± 1. 4 31. 7  ± 1. 0 40. 3  ± 0. 3 21. 9  ± 0. 5 25. 6  ± 0. 7 28. 9  ± 1. 3 Pa 1659 25. 7  ± 0. 6 + + 20. 5  ± 0. 1 21. 4  ± 0. 8 25. 8  ± 0. 1 26. 7  ± 0. 5 + + 50. 2  ± 1. 2 56. 5  ± 0. 8 60. 2  ± 0. 3 40. 6  ± 0. 4 45. 3  ± 0. 8 56. 2  ± 0. 7 30. 2  ± 1. 1 48. 5  ± 0. 6 59. 2  ± 0. 1 24. 3  ± 0. 4 26. 3  ± 1. 5 27. 3  ± 1. 1 Leaf oleoresin Eugenol Bark volatile oilBark oleoresin E-cinnamaldehyde Ampicillin Bs = Bacillus s ubtilis; Sa = Staphylococcus aureus; Bc = Bacillus cereus ; Ec = Escherichia coli ; St = Salmonella typhi; Pa = Pseudomonas aeruginosa. (+) indicates complete inhibition. a Average of three replicates. level of 500 lg mLA1. Another report (Smith-Palmer et al. , 1998) found the MICs of C. zeylanicum against E. coli and S. aureus were 0. 05% and 0. 04%, respectively. To con? rm the relationship of the constituents in cinnamon leaf and bark and antimicrobial activity, the major components were tested for antimicrobial activity. The results are shown in Tables 3–5.Among both constituents, E-cinnamaldehyde possessed better activity and these ? ndings are quite similar with the results of Chang et al. (2001). However, eugenol, in spite of being phenolic compound, failed to inhibit the fungal growth by inverted petriplate method but when it was added directly to the growth media in higher concentrations, it appeared to inhibit completely the microbial growth. Nevertheless, it is wor th noting that essential oils and oleoresins are very heterogeneous mixtures of a single substances, biological actions are primarily due to these components in a very complicated concert of synergistic or antagonistic e? cts. Table 6 Comparison of scavenging e? ects of cinnamon leaf and bark volatile oils and oleoresins against DPPH and hydroxyl radicals Sample Radical scavenging activitya (%) DPPH radical 5 lL Leaf oil Leaf oleoresin Eugenol Bark oil Bark oleoresin E-cinnamaldehyde BHA BHT a Hydroxyl radical 15 lL 69. 9 74. 1 65. 2 76. 2 89. 3 72. 3 92. 1 89. 2 20 lL 72. 1 76. 7 71. 3 82. 1 91. 2 75. 1 94. 7 91. 7 25 lL 73. 9 91. 2 92. 9 83. 6 95. 3 78. 3 96. 4 94. 9 5 lL 31. 2 43. 6 39. 4 51. 2 57. 6 49. 8 71. 3 66. 2 10 lL 55. 7 57. 1 45. 1 57. 6 62. 3 53. 6 75. 1 72. 1 15 lL 63. 5 70. 4 54. 3 73. 1 68. 9 57. 1 78. 75. 3 20 lL 68. 1 73. 6 61. 5 76. 9 71. 2 65. 2 81. 7 77. 5 25 lL 72. 2 86. 1 68. 2 79. 6 78. 6 68. 3 84. 9 83. 2 10 lL 58. 7 58. 9 56. 8 73. 5 87. 5 68. 1 89. 3 85. 1 45. 2 51. 3 41. 3 71. 1 75. 6 65. 3 78. 4 81. 2 Average of three replicates. 1660 G. Singh et al. / Food and Chemical Toxicology 45 (2007) 1650–1661 Chang, S. T. , Chen, P. F. , Chang, S. C. , 2001. 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(Eds. ), Phenolic Compounds in Food and Their E? ects on Health.American Chemical Society, New York, pp. 54–72. 4. Conclusion The present study provided the potential antimicrobial and antioxidant properties of the volatile oils and oleoresins of cinnamon leaf and bark. The oleoresins have shown better e? ect on primary and secondary oxidation products in mustard oil. The radical scavenging activity and other complementary assays are also in good correlation. Moreover, the potency of the constituents such as eugenol and cinnamaldehyde could provide a chemical basis for some of the health bene? ts claimed for cinnamon and warrant further studies to assess their potential as e? ctive natural remedies. Acknowledgements We are thankful to Head, Chemistry Department, DDU Gorakhpur University, Gorakhpur for provi ding laboratory facilities. Prof. K. D. S. Yadav of our department is also thanked for providing spectral facility. 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Personal Characteristics of Nick Thomas, Charles Henry, and Chairman Lup In Wisdom Sits in Places

The significance of place-names is compounded in the personal characteristics of Nick Thompson, Charles Henry, and Chairman Lup. Although their personal characteristics differ significantly in terms of attitudes and approach to reality, their behavioral orientation are relatively the same. The subscription to a single set of norms (the rules attached to the place-names) ensures a relatively predictable pattern of behavior from the three characters.Hence, one can assume that the behavioral orientation of the three characters stemmed not from their intrinsic characteristics but rather to their personal interpretations of ‘reality. Here, reality takes the form of adjunctive reference to the past, the legacy of place-names. There is a need, however, to look into the personal characteristics (behavioral) of the characters to provide credence to the above-mentioned proposition. Charles is a traditionalist, an individual who clings to the general beliefs and rules of ancient customs. His minimal exposure to the vagrancies of modern life did not affect his behavioral orientation towards customs and tradition. In fact, most of his actions indicate a persona that embraces the beauty and glorious existence of place-names.In page 10 of the book, Charles said to Mosley: What he’s doing isn’t right. It’s not good. He seems to be in a hurry. Why is he in a hurry? It’s disrespectful. Our ancestors made this name. They made it just as it is. They made it for a reason. They spoke it first, a long time ago! He’s repeating the speech of our ancestors. He doesn’t know that. Tell him he’s repeating the speech of our ancestors. Charles’ obsession with mental images and speeches of his ancestors created a personal impression of sturdiness in his character.He refuses to allow changes that will affect his beliefs on place-names. Nick Thompson behavioral orientation is similar to that of Charles Henry. An old man who delighted in telling humorous and often embarrassing stories, his expression is quite mischievous and intimidating. In the tribe though, he is known as the true â€Å"Slim Coyote. † Thompson’s character can be described in four words: serious, generous, intelligent, and outspoken. In anthropology, the character of Nick Thompson exemplifies the character of an ideal elder who teaches the younger generation the essence of existence.In page 43 of the book, he said, â€Å"Start with the names. I will teach you like before. Come back tomorrow morning†¦ White men need paper maps. We have maps on our minds. † Due reference again is made to the highly revered place-names. The Western Apache’s reverence to place-names are generally rooted from the historical value of the place-names themselves. This historical value is imbued in the personal beliefs of every Apache. It can be said that Nick Thompson, by virtue of his age, has strong personal attachment to this histori cal value.In fact, his understanding bear more influence to his behavioral orientation than his actual belief in the place-names themselves. His motto in life is: â€Å"Learn the names† (a deep understanding of the place-names). Chairman Lup’s character is generally similar to that of Thompson. His obsession with stories about place-names is the primary foundation of his behavioral orientation. For the most, an understanding of his character forces one to assume that he is a strict traditionalist. His personal belief about place-names is actually greater than Charles Henry and almost equal to that of Nick Thompson.